Journal: bioRxiv

Article Title: MicroRNA-195-3p as a potential regulator of hypoxic injury in HUVECs

doi: 10.1101/2023.02.23.529661

Figure Lengend Snippet: miR-195-3P inhibitor regains hypoxia-induced HUVECs proliferation rate. ( A, B ) EdU results shows the proliferation rate of each group. The cells were stained with Hoechst 33342 (5 μg/mL), and the proliferation population was analyzed (n=3). Scale bar, 50 μm. ( C ) The viabilities of cells in the different treatment groups was measured using the CCK-8 assay (n=5). ( D, E ) Representative images of cell colonies. Colony formation assays determined cell proliferation in HUVECs. **p < 0.01, ***p < 0.001 vs. the control group. ##p < 0.01, ###p < 0.001 vs. hypoxia induction for 6 h group. HUVECs, human umbilical vein endothelial cells; EdU, 5-ethynyl-2’-deoxyuridine; CCK-8, Cell Counting kit-8.

Article Snippet: HUVECS viability was assessed by Cell Counting Kit-8 (CCK-8, Dojindo, Japan) assay as described previously.

Techniques: Staining, CCK-8 Assay, Control, Cell Counting

Journal: Drug Delivery

Article Title: In silico identification and experimental validation of cellular uptake and intracellular labeling by a new cell penetrating peptide derived from CDN1

doi: 10.1080/10717544.2021.1963352

Figure Lengend Snippet: Cytotoxicity evaluation on peptide P2. (A) Cell viability assessment of HeLa cells incubated with the indicated concentration of peptide P2 after 24 and 48 hours. All measurements (3 replications of each group) were normalized to the protein concentration of cell lysate, and error bars represent S.E.M., the two‐way analysis of variance (ANOVA) with Bonferroni’s multiple comparison test was used to compare the differences. (B) Cell viability assessment of HepG2 cells incubated with the indicated concentration of peptide P2 after 24 and 48 hours. All measurements (3 replications of each group) were normalized to the protein concentration of cell lysate, and error bars represent S.E.M., the two‐way analysis of variance (ANOVA) with Bonferroni’s multiple comparison test was used to compare the differences. (C) LDH release assay of HeLa cells incubated with the indicated concentration of peptide P2, 0.1% Triton X-100 was used as a positive control. The baseline indicated in the plot represents negative control. (D) LDH release assay of MCF7 cells incubated with the indicated concentration of peptide P2, 0.1% Triton X-100 was used as a positive control. The baseline indicated in the plot represents negative control.

Article Snippet: Human breast cancer cell line MCF7 (P 20), human lung cancer cell line A549 (P 20), human cervical cancer cell lines HeLa (P 20), human hepatocellular carcinoma derived HepG2 (P 20), and rat hepatic stellate cell line T6 cells (P 20) were ordered from ATCC (American Type Culture Collection) and stored in our lab. All these cell lines were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% heated-inactivated fetal bovine serum (FBS), penicillin (100 U/ml) and streptomycin (0.1 mg/ml).

Techniques: Incubation, Concentration Assay, Protein Concentration, Comparison, Lactate Dehydrogenase Assay, Positive Control, Negative Control